The course is designed to cover the Players of Gene cloning, Various Strategies for Gene cloning and expression of cloned gene in different Expression system, (E. coli, Insect cell, Plant, animal cell) Techniques used in Recombinant DNA tech, Protein/ protein and protein/DNA interaction, Different types of PCR, Basic chemistry and math, identification of gene function, Basics of vaccine, Protein Engineering, Genetic engineering in plants. The question Answers session for each topic will help participants to solve the questions of CSIR-NET, ICMR-JRF, GATE, CUCET, GAT-B etc.